The purpose of this study is to examine the teacher perceptions, practices and challenges of differentiated instruction in School. To know the perception of teachers on differentiated instruction, the methodology that I will use in this study is a quantitative primary data. For a quantitative study, to get the best results, for collecting data I will do interviews, and observations. I have select 25 different subject primary school teachers and 25 parents from Ihaddhoo School. The study allows me to explore more about differentiated instruction and teachers’ interest levels in providing differentiated instruction in their lessons. This study helps me to explore the teacher perceptions, practices and challenges of differentiated instruction.